Fill The Tubes In The Order Of Draw Recommended By Clsi To Prevent Contamination. (2023)

FAQs

Fill The Tubes In The Order Of Draw Recommended By Clsi To Prevent Contamination.? ›

CLSI recommends the following order of draw for skin puncture: Blood gases, EDTA tubes, Other additive tubes, then Serum tubes. The correct order of draw would be: two light blue top tubes (one waste and one for PT which is a coagulation test), green top tube (for the Chem 7), and the lavender top tube (for the CBC).

Clinical & Laboratory Standards Institute (CLSI) Guidelines

The guidelines cover order-of-draw recommendations, which continue to be present in the guidelines as blood culture first, followed by citrated tubes, serum tubes, heparin, then EDTA, with special emphasis on not collecting the EDTA tube before the serum tube.

The most current Clinical and Laboratory Standards Institute (CLSI) guidelines recommends a single "Order of Draw", whether using a multisample, evacuated tube system or drawing with a syringe(s). The "Order of Draw" is designed to eliminate the possibility of cross contamination that may result in erroneous results.

The correct order of draw follows: Blood culture tube or bottle. Sodium citrate tube (eg, blue closure) Serum tubes, including those with clot activator and gels (eg, red, red-speckled, gold closures)

Nowadays the order of blood draw established by the international guidelines is postulated to avoid cross-contamination between different additives contained in blood collection tubes, and it is as follows: blood culture bottle, non-additive tube, coagulation tube, clot activator, serum separator tube, sodium heparin, ...

The benchmark of blood contamination rate is <3%, as proposed by the Clinical Laboratory Standard Institute (CLSI) (5).

The CLSI standards recommend cleansing the site with friction using a clean gauze pad with 70% isopropyl alcohol solution or a commercially prepared alcohol pad.

The Clinical and Laboratory Standards Institute (CLSI) is a not-for-profit organization that develops laboratory standards worldwide. Our standards are recognized by laboratories, accreditors, and government agencies as the best way to improve medical laboratory testing.

Clinical and Laboratory Standards Institute.

CLSI's library of Microbiology standards and AST guidelines establish vital performance criteria and best practice guidelines for use in the clinical microbiology laboratory field.

What is the correct order of draw with tubes? ›

The recommended order is as follows: Blood culture tubes. Sodium citrate tubes (e.g., blue-stopper) Serum tubes with or without clot activator, with or without gel separator (e.g., red-, gold-, speckled-stopper)

NOTE: All tubes sterile. Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first.

Clinical and Laboratory Standards Institute (CLSI) subscribes to a quality management system (QMS) approach in the. development of standards and guidelines, which facilitates project management; defines a document structure using a. template; and provides a process to identify needed documents.

2. Blue Top – Tube contains Sodium Citrate. Most often used for coagulation studies. Must be drawn prior to collection of serum tubes to prevent contamination with clot activator and interference with coagulation cascade.

Note: When multiple specimens are drawn from a single venipuncture, the following order is recommended: (1) sterile blood culture tubes, (2) nonadditive clotting tubes (red), (3) coagulation tubes and tubes containing citrate (blue), (4) gel-barrier tubes and tubes with additives (red), (5) tubes containing heparin ( ...

According to the CLSI recommended order of draw for capillary puncture, which of the following micro collection tubes must be collected first? The potassium EDTA micro-collection tube should be collected first because it is used for whole blood tests and micro clots easily in these containers from inadequate mixing.

Prevent Cross Contamination

The reason the tubes are assigned this order is to prevent the cross-contamination of chemicals into other tubes which may cause errors in testing. This process is called reflux, which means the chemical can move from the tube and enter the needle.